ALEXYS Neurotransmitter Analyzer for On-line Microdialysis Sampling
ثبت نشده
چکیده
Electrochemistry Discover the difference Summary The ALEXYS Neurotransmitter Analyzer is a modular system for UHPLC with electrochemical detection of neurotransmitters. This application note shows the applicability of the system to measure various biogenic amines in on-line microdialysis (OMD) which is a continuously flowing microdialysis sampling setup. Instead of collecting samples and putting them in an autosampler, the sample is collected in a sample loop and injected into the system for immediate analysis. During analysis the next sample is already being collected in the loop and the process repeats continuously. Different set-ups are highlighted for improved time resolution, high throughput, and a parallel setup for multicomponent analysis (biogenic amines and metabolites). n Immediate feedback on the microdialysis experiment n Multi component analysis in parallel UHPLC n High time resolution n High throughput, up to 4 simultaneous experiments ALEXYS Neurotransmitter Analyzer for On-line Microdialysis Sampling
منابع مشابه
Ethylene Glycol in Hydroxyethyl Starch According to EP
The impurity analysis of ethylene glycol in hydroxyethyl starch (HES) was evaluated using the exact method and conditions described in the official EP 8.2 (2014) monograph [2]. The evaluation was performed using an ALEXYS® LC-EC analyzer for ethylene glycol, based on the DECADE Elite electrochemical detector and a SenCell with Au working electrode (WE) and HyREF (Pd/H 2 ) reference electrode. D...
متن کاملEffect of bupropion on hippocampal neurotransmitters and on peripheral hormonal concentrations in the rat.
The purpose of the present study was to administer an acute dose of the dual dopamine norepinephrine reuptake blocker bupropion in freely moving rats and to monitor the extracellular neurotransmitter concentrations in the hippocampus via in vivo microdialysis and the peripheral hormonal concentrations via catheterization. A microdialysis probe was inserted in the hippocampus, and samples for se...
متن کاملContinuous in vivo monitoring of blood diffusible calcium using on-line microdialysis sampling coupled with flame atomic absorption spectrometry.
A direct, rapid and continuous in vivo monitoring of diffusible calcium in the blood of living rabbits has been developed using microdialysis sampling coupled on-line with flame atomic absorption spectrometry. Microdialysates perfused through implanted microdialysis probes were collected with a sample loop on an injection valve and directly introduced into the flame atomizer by a carrier soluti...
متن کاملModeling and Simulation of Microdialysis in the Deep Brain Structures
Microdialysis is a method for monitoring of the local biochemical environment in a region of interest. The method uses a catheter, mimicking the function of a blood capillary, to sample substances from the surrounding medium through diffusion. A recent application for microdialysis is the sampling of neuroactive substances in the deep brain, or basal ganglia, during deep brain stimulation (DBS)...
متن کاملValidation of subcutaneous microdialysis sampling for pharmacokinetic studies of flurbiprofen in the rat.
The objective of this study was to validate subcutaneous (sc) microdialysis sampling to study flurbiprofen pharmacokinetics and plasma protein binding in the awake freely moving rat. A linear microdialysis probe was manufactured using a Hemophane hollow fiber which was tested in vitro and in vivo for the recovery of flurbiprofen and naproxen used as retrodialysis marker. Flurbiprofen was admini...
متن کامل